Generator

Part:BBa_K1216005:Design

Designed by: Parvathi Chandran   Group: iGEM13_ETH_Zurich   (2013-08-29)


Alkaline phosphatase (phoA) from Citrobacter with TEV and poly-HIS tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1507
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

add a his tag


Source

E.coli

References